Proteolysis of sialoglycoprotein by Pasteurella haemolytica cytotoxic culture supernatant
نویسندگان
چکیده
منابع مشابه
The production and evaluation of Pasteurella haemolytica leukotoxin in the supernatant of submerged cultures in fermenters.
The optimal production of P. haemolytica leukotoxin in the culture supernatant of a fluid medium is dependent on a number of factors. The leukotoxin has to be produced by using a strain that is known for its ability to produce high quantities of leukotoxin, inoculated into the most suitable type of medium at the correct culture density containing the necessary supplements and harvested after a ...
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In this study, we used an in vitro coculture system to determine which virulence factor from Pasteurella haemolytica Al was responsible for augmenting bovine polymorphonuclear neutrophil (PMN)-mediated killing of bovine pulmonary artery endothelial cells (BPAEC). A 5"Cr release cytotoxicity assay was used as a measure of BPAEC killing. The mechanisms associated with this BPAEC killing were also...
متن کاملPasteurella haemolytica A2
Twenty-one healthy local goats of about eight months old were divided into four groups consisting of six animals in groups 1, 2 and 3 and three animals in group 4. Goats in groups 1 and 2 were inoculated intratracheally with Mycoplasma arginini. Goats in group 2 were inoculated again with Pasteurella haemolytica A2 six days later. Goats in group 3 were inoculated intratracheally with P. haemoly...
متن کاملTaxonom ic relationships of the [ Pasteurella ] haemolytica
3 National Institute of Public Health, Department of Bacteriology, PO Box 4404 Torshov, 0403 Oslo, Norway The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the t re ha loseneg a t ive [Pasteurella] haemolytica com p I ex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 165 rRNA sequencing a...
متن کاملActivation of bovine neutrophils by partially purified Pasteurella haemolytica leukotoxin.
In this study we developed a new method for the partial purification of Pasteurella haemolytica leukotoxin by size-exclusion high-performance liquid chromatography. The partially purified leukotoxin had a molecular weight of 104,000, as estimated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and reacted on an immunoblot with an antileukotoxin monoclonal antibody. As expect...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1983
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.42.1.64-70.1983